The impact of molybdenum on xanthine oxidase and its associated activities in ovine milk
Аннотация
Xanthine oxidase is a molybdenum and iron containing flavoprotein, catalyzing the final oxidation stage of purines and oxidative transformation of pterins and some aliphatic and aromatic aldehydes.
The results show that in fresh sheep milk xanthine oxidase does not contain molybdenum. Apparently, XO located in the inner membrane of the fat globule micelles (MFGM) is not available for exogenous molybdenum. During heat treatment at 80 °C, the milk globules are destroyed, then the molecule is denatured. As a result, the access of molybdenum to the MPT (‘molybdopterin′ or metal-binding Pterin ene-1,2-dithiolate)-containing active center increases. Our and other numerous studies have shown that MPT is extremely sensitive to oxygen.
Despite the importance of this enzyme, the distribution of xanthine oxidase in traditional household animal tissues is unknown. Formerly, we have found most of the xanthine oxidase molecules in animal milk are inactive because of lack of molybdenum. Ovine milk was processed by inserting in vivo molybdenum in drinking water. Heating the milk of animals at 80 °C for 5 minutes in the presence of molybdenum and cysteine led to a sharp increase of xanthine oxidase and it’s associated - nitrate reductase and nitrite reductase activities.
Автор
A. S. Mukhamejanova*
Z. А. Alikulov
DOI
https://doi.org/10.48081/PYRD2567
Ключевые слова
sheep, milk
molybdenum
xanthine oxidase
nitrate reductase
nitrite reductase
activity
Год
2022
Номер
Выпуск 2
Для цитирования:
A. S. Mukhamejanova*, Z. А. Alikulov The impact of molybdenum on xanthine oxidase and its associated activities in ovine milk // Научный журнал «Наука и техника Казахстана» - 2022 - №2 - Р. 88–94. https://doi.org/10.48081/PYRD2567
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